Jordan Thompson retires from Wimbledon round-of-16 match with American Taylor Fritz

Australian Jordan Thompson's best Wimbledon run has ended in heartbreak as he was forced to retire from his round-of-16 match against Taylor Fritz.

Thompson, who came to the All England Club with a back issue, called for a medical timeout midway through the second set due to an apparent right thigh problem and, after three more points, decided to call it a day while trailing 1-6, 3-0.

Both players played five-setters in rounds one and two, and four in their previous matches, but Thompson looked to be feeling the effects of his 10 and a half hours on court as Fritz raced through the first set.

Thompson was broken with little fuss in the opening game of the second and it was clear the 31-year-old was struggling with his movement.

The medical timeout after Fritz clinched another break only delayed the inevitable as the match, Thompson's first in the fourth round at SW19, was soon brought to a premature end.

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"Not the way I wanted to go through, I was excited to play Jordan today, I was excited to play good tennis," Fritz said.

"It's sad … he's been battling out here, playing five-setters, I respect him for coming out … I feel bad for him and I hope he gets better."

The result means Alex de Minaur is the last remaining Australian in either the men's or women's singles draw.

De Minaur faces Novak Djokovic, fresh off his 100th win at Wimbledon, in the round of 16 on Monday evening AEST.

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Reuters/ABC

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